RNA-seq-based Transcriptome Analysis of A LuxR-like (DqsR) Mutant of Deinococcus radiodurans Under Oxidative and Non-oxidative Stresses.
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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Purpose: The goal of this study is to explore the role of DqsR of D. radiodurans R1 in transcriptome profiling under oxidative and non-oxidative stresses.Methods: mRNA profiles of wild-type D. radiodurans R1 and DqsR knockout strain with 100 mM H2O2 treatment or not were generated by deep sequencing using IIllumina Solexa.Results: A total of 17.03 million (M) and 19.61 M clean reads were obtained from the wild-type R1 and mutant strains; of these, 14.81 M and 16.78 M reads were mapped to the genome, respectively. A total of 427 genes were repressed and 396 genes were induced at least one-fold (log2) (p<0.05) in the mutant under non-oxidative stress conditions in transcription.Conclusions: Our study reveals that deletion of DqsR changes the transcriptional levels of a number of genes, both under normal conditions and oxidative stresses, including genes involved in translation, transcription, replication, recombination and repair, inorganic ion transport and metabolism, etc.
提供机构：
Zhejiang University
创建时间：
2022-02-20
