Identification of the intracellular protein targets of a clickable half-sandwich iridium complex by chemical proteomics

This study aims at identifying intracellular proteins that are targeted by half-sandwich Ir(III) organometallic drugs. We recently demonstrated that half-sandwich Ir(III) complexes are able to form adducts with the side chain of certain amino-acids (Cys and His for example) and with cellular proteins in vivo. We use an azido-substituted half-sandwich iridium complex to fish out protein adducts, using click chemistry and ethynyl-agarose beads