Additional file 1 of A simple, precise, and sensitive HPLC method for quantification of letrozole in rat plasma: development, validation, and preclinical pharmacokinetics

Additional file 1. Method optimization – effect of mobile phase compositions and internal standard (IS). Fig. S1. Effect of mobile phase composition and PA (IS). Chromatograms of a) diluent, b) LTZ, c) PA, d) blank plasma and e) spiked plasma using ACN and acetate buffer pH 4.5 (50:50 %v/v) at 0.8 mL/min. Fig. S2. Effect of mobile phase composition and KCZ (IS). Chromatograms of a) diluent, b) LTZ, c) KCZ, d) blank plasma and e) spiked plasma using ACN and phosphate buffer pH 5.5 (65:35 %v/v) at 0.8 mL/min.