mRNA of pacific white shrimp under acute alkalinity stress

Transcriptome sequencing can both analyze the genes expression and reveal its regulatory mechanism, and miRNAs usually bind to partially complementary 3' untranslated regions of target mRNA in aquatic animals and regulate the translation of target genes. The recent studies can effectively reveal the expression patterns and regulation mode of genes under environmental stress through miRNA-mRNA analysis. Transcriptome sequencing and miRNA-mRNA targeting relationship analysis can interpret complex life processes and provide a powerful tool for interpreting the complex molecular response mechanism. Therefore, this study screened candidate genes and related pathways for alkalinity stress through transcriptome sequencing and miRNA-mRNA combined analysis, and explored the expression profiles of miRNA and miRNA-mRNA related to alkalinity stress. This research provides an important theoretical basis for interpreting the molecular response mechanism of pacific white shrimp under alkaline environment, and also provides scientific basis for the optimisation of existing crustaceans aquaculture technology.