Epithelial-mesenchymal transition markers screenedina cell-based model and validated in lung adenocarcinoma

The epithelial-to-mesenchymal transition (EMT) process in cancer enables the migratory and invasive capabilities associated with metastatic competence. The cancer microenvironment plays an important role in inducing the occurrence of EMT. A number of extracellular stimuli and associated signaling pathways have been identified to promote EMT, however, molecular features during the early stages of EMT are poorly understood. Here we have established a novel in vitro EMT-inducing system that mimi cs tumor microenvironment and allows for close monitoring of the EMT progress. Using RNA-seq and miRNA-seq, we analyzed the transcriptomic transitions accompanying the progression of EMT, and pinpointed several molecular features during early EMT of the lung carcinoma cells. Overall design: CAF conditioned medium treated and control A549 cells were collected at six different time points (3h, 6h, 12h, 24h, 48h, 72h) for RNA-seq and miRNA-seq, two biological replicates for each sample.