Dataset for Enzyme synthesis and continuous flow separation of (R)-1-phenylethanol in a modular microfluidic system
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______Dataset for Enzyme synthesis and continuous flow separation of (R)-1-phenylethanol in a modular microfluidic system___________ ______Contact______* Michal Pribyl* pribylm@vscht.cz* +420 220 444 445* ORCID: 0000-0002-8288-9127* Dept. of Chemical Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague* Technicka 5, 166 28, Prague 6, Czech Republic ______Data manager or custodian______* Lukas Sauer* sauerl@vscht.cz* +420 220 443 101* ORCID: 0000-0002-8911-9299* Dept. of Chemical Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague* Technicka 5, 166 28, Prague 6, Czech Republic ______Licence______* Dataset for Enzyme synthesis and continuous flow separation of (R)-1-phenylethanol in a modular microfluidic system by Lukas Sauer is licensed under CC BY 4.0* licence information: https://creativecommons.org/licenses/by/4.0/------------------------------------------------------------------------------------------------ ______About the dataset______* The dataset contains peak areas of phenylethanol, phenylethyl acetate read off from HPLC chromatograms, which were subsequently converted to concentration. Our study aims to develop a functional modular microfluidic system capable of continuously producing an industrially significant enantiomer (R)-(+)-1-phenylethanol (R-PE) from racemic 1-phenylethyl acetate (PEAc). The system consists of a packed-bed reactor (PBR) with an immobilized lipase, which synthesizes R-PE, and a slug flow extractor (SFE), where the unreacted substrate is removed from the reaction solution using hexane. ______Methods of data collection______* HPLC analysis:Samples taken from the reactors were analyzed in the HPLC system Agilent 1260 equipped with a UV-VIS detector and a chiral column Lux Cellulose-1 (Phenomenex). The absorbance was measured at the wavelength of 220 nm. ______Methods of data processing______* There is a lot of raw data from HPLC, and special software (OpenLab CDS) is needed to open it. HPLC software allows data from the chromatograms to be exported to an XLSX file. Raw data exported from chromatograms are denoted as “A (R,S-PE)” and “A (R,S-PEAc)”, which are peak areas of (R), (S)-(+)-1-phenylethanol and (R), (S)-(-)-1-phenylethyl acetate read off from the HPLC chromatogram. These peak areas were then recalculated to concentrations using calibration. The XLSX files were then converted to CSV.
