Identify targets of miRNAs involved in ER stress in wheat using degradome sequencing

We used dithiothreitol (DTT) and tauroursodeoxycholic acid (TUDCA) to induce or suppress ER stress in wheat cells, respectively, with the aim to reveal the molecular background of ER stress responses using degradome sequencing. After combined analysis of high-throughput sequencing, a total of 12,453 miRNA-target pairs were verified by degradome sequencing, and among them, 7,484 genes were targeted by 1,233 miRNAs. Experiments were established with three treatments: control (C), DTT (D), and DTT+TUDCA (T). Three independent biological replicates were performed, and a total of nine leaf samples were performed by degradome sequencing.