RNA-Seq analysis of 9G8-RNAi intestines in Drosophila

Previous studies from our lab have characterized a group of splicing factors called SR proteins that function to identify intron/exon borders in regulating metabolic homeostasis in the Drosophila fat body. Decreasing the function of one SR protein, 9G8, causes an increase in triglyceride storage; however, the function of 9G8 in other metabolic tissues like the fly intestine is not known. Decreasing 9G8 in the Drosophila intestine results in the accumulation of triglycerides; however, the 9G8 target genes responsible for this phenotype are unknown. To address this question, we performed RNA sequencing on Drosophila intestines with 9G8 levels reduced by RNAi. Overall design: Drosophila intestine mRNA profiles of yolk-Gal4>9G8-RNAi intestines compared to yolk-Gal4>GFPdsRNA control fat bodies (3 samples of each genotype).