Data Sets of ESR1 Expression in HESC cultures and ESR1 and PR levels in Wistar rat's endometrium when treated with Adansonia digitata root bark fractions.

Adansonia digitata Linn root bark extract alters oestrogen and progesterone activities in the female Wistar rat. We hypothesized that components of the extract may act through the regulation of oestrogen and progesterone transduction. We obtained fractions from Aqueous methanol extract of Adansonia digitata root bark through Column chromatography with different solvents; acetyl acetate, methanol, and distilled water, to yield acetyl acetate-methanol fraction (AAMF); Aqueous methanol fraction (AQMF); and Aqueous fraction (AQF). The fractions were investigated for their effect on the gene expression of oestrogen receptor alpha (ESR1) in Human endometrial stromal cell (HESC) line (Data 1).
The effect of the acetyl acetate-methanol fraction of the extract on the location and levels of ESR1 and progesterone receptor alpha-beta (PR) in the mature virgin female Wistar rat's uterus was also investigated (Data 2 - 6).
The results revealed that AAMF at 50 – 500 µg /ml downregulates ESR1 gene expression in HESC cultures. In the Wistar rat's uterus, AAMF at 150 - 300 mg/kg bodyweight caused a reduction in membrane-located ESR1 levels in the endometrial glands and epithelium, but increased PR levels in the endometrial stroma.