A human maternal-fetus interface transcriptome atlas

Trophoblasts undergos dynamic differentiation and self-renewal during pregnancy. Expecially, EVTs anchor the placenta to the uterus to ensure pregnancy success, and its invasive excessiveness is a probable consequence of ruptured tubal ectopic pregnancy (REP), that resulting in maternal infertile and/or even deaths. We present REP, abortive tubal ectopic pregnancy (AEP)and intrauterine pregnancy (IP) atlas by integrating single-cell RNA-seq. We define cell subtypes within EVTs and each of the feature genes. Further, we utilize monocle3 and RNA velocty to identify the developmental trajectories of EVT subsets. CellPhoneDB and cell-chat unravel secretion of CSF1 by falloppian tubal secretory epithelial cells directly interact with CSF1R of EVT to strengthens the invasive ability. Overall design: Expression profiling by high throughput sequencing