DK2, a newly synthesized chalcone, induced apoptosis and differential expression profiles of miRNA and mRNA on breast cancer MCF-7 cell line

In this study, the cytotoxic effect of DK2, a newly synthesized chalcone and its underlying mechanism against breast cancer cell line, MCF-7 were investigated. MCF-7 was treated with 8.2 μM of DK2 at several time points. The apoptotic analysis was carried out using MTT assay, morphology observation (using inverted microscope and fluorescent microscope) and flow cytometry analysis (using Annexin V/7ADD apoptosis analysis and cell cycle analysis). Triplicate of DK2-treated MCF-7 cell and control untreated MCF-7 were subjected to miRNA and mRNA microarray profiling (Affymetrix, USA).