IC50 values of the diminazene aceturate drug evaluated for B. bovis and T. equi by fluorescence- and microscopy-based methods without daily replacement of the medium with the optimal HCTs.

a IC50 values for each HCT were calculated on the fourth day based on the growth inhibitions determined by fluorescence- and microscopy-based methods in three separate experiments. Each drug concentration was made in triplicate in each experiment, and the final obtained IC50s were the mean of three obtained values from separate experiments.b The differences between IC50 values calculated by fluorescence- and microscopy-based methods for the optimal HCTs.IC50 values of the diminazene aceturate drug evaluated for B. bovis and T. equi by fluorescence- and microscopy-based methods without daily replacement of the medium with the optimal HCTs.