Osteoblastic loss of Ezh2. Osteoblastic loss of Ezh2
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA436340
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资源简介：
Osteblastic loss of Ezh2 was achieved by breeding Ezh2flox/flox (Su et al., PMID: 12496962) with Osx-Cre (Rodda et al., PMID:16854976). Overall design: Calvaria were dissected out from 2 to 3 day old wild type (WT, Ezh2_WT_Osx) and Ezh2 conditional knockout (Ezh2 cKO, Ezh2_cKO-Osx), washed in PBS, digested for 20 minutes in collagenase digestion medium (αMEM, 2mg/ml Collagenase type II, and 0.005% trypsin) at 37oC, washed in PBS, and snap frozen with liquid nitrogen and stored at -80oC. RNA was then isolated utilizing Ultra Turrax T25 (IKA) and Direct-zol™ RNA kit (Zymo Research).
创建时间：
2018-02-28
