Influence of Dnmt3a knockout on gene expression of preleukemia B cell and CLL cells

Although somatic mutations in the DNA methyltransferase 3A (DNMT3A) in chronic lymphocytic leukemia (CLL) are rare, DNMT3A transcript and protein expression is frequently reduced in CLL patients, and previous studies have associated low DNMT3A expression with more aggressive disease and poorer survival. Overall design: Here we generate B cell-restricted conditional knockout of Dnmt3a in mice and used RNA-seq and to investigate its influence on gene expression in preleukemia B cells and CLL cells.