Characterisation of TET function in drosophila larval central nervous system

Enzymes of the Ten Eleven Translocation (TET) family play a key role in the regulation of gene expression in many species by oxidizing 5-methylcytosine (5mC), a prominent epigenetic mark, into 5-hydroxymethylcytosine (5hmC). Yet, TET proteins also have non-canonical modes of action beyond 5mC oxidation, notably in Drosophila, whose genomes is devoid of 5mC. Here, we used a combination of NGS analyses to studied the funciton and mode of action of Tet in the central nervous system of Drosophila larvae. All experiments were performed with biological replicates (duplicates for ChIP-seq and CUT&RUN; quadruplicates for RNA-seq). w1118 (wt)were used as control lines.