TNF-α released by melanoma liver metastasis activates AKT and NFKβ1 pathways to promote collagen type I secretion by hepatocytes. TNF-α released by melanoma liver metastasis activates AKT and NFKβ1 pathways to promote collagen type I secretion by hepatocytes

A total of four human melanoma liver metastasis (MLiM) formalin-fixed paraffin-embedded (FFPE) sections were analyzed using Nanostring GeoMx digital spatial profiling (NGDSP). The FFPE tissues were obtained from patients who underwent surgery and were pathological diagnosed with MLiM. Patients who had received any preoperative therapy were excluded. In each region of interest (ROI), two areas of interest (AOI)s were defined by HepPar1+ (hepatocytes); HMB45+ (melanoma cells) staining. A total of 43 AOIs were carefully reviewed for segments with sequencing saturation 1.5, <-1.5, p < 0.05) were calculated on AOIs selected from MLiM comparing TC vs. TM; ANH vs. DNH; and ANH vs. Melanoma cells (TC+TM). Overall design: Four FFPE tissue samples were analyzed by NanoString GeoMX Digital Spatial Profiling. The regions of interest (ROI) were collected in each tissue samples and segmented in areas of interest (AOI) based on the staining pattern of two fluorescently labeled cell type specific morphological markers: HepPar1+ and HMB45+.