series5_GSE147507 : Differential gene expression of A549 Cells_ SARS-CoV-2 (MOI 2.0) infected vs. Mock control

Differentially expressed genes between SARS-Cov-2 (MOI 2.0) infected vs Mock control from A549 cells (series5_GSE147507) with fold change, raw counts, and normalized counts for each gene. <br>RNA-Seq reads (FASTQ) of biological triplicates from A549 cells, mock treated or infected with SARS-CoV-2 (USA-WA1/2020) were downloaded from NCBI SRA and data analysis was performed using CLC Genomics workbench (CLC v20). The reads were aligned to the human genome (hg38) with annotations (Ensemble v91) and then quantification and differential expression of genes between SARS-Cov-2 infected vs. mock control samples was performed. The differentially expressed genes were filtered by FDR P-value&lt;0.05 and uploaded here. <br><br><br><br><br><br><br><br><br><br><br> <br>

本数据集包含A549细胞（series5_GSE147507）中，以感染复数（Multiplicity of Infection, MOI）2.0感染新冠病毒（SARS-CoV-2）的实验组与Mock对照组之间的差异表达基因，并附带每个基因的倍数变化、原始计数与标准化计数信息。

研究人员从NCBI序列读取档案（NCBI Sequence Read Archive, NCBI SRA）下载了A549细胞的三份生物学重复样本的RNA测序读段（FASTQ格式），这些样本分别经Mock处理或感染新冠病毒USA-WA1/2020毒株，并使用CLC基因组工作台（CLC Genomics workbench, CLC v20）完成数据分析。将测序读段比对至带有注释的人类参考基因组hg38（Ensemble v91）后，研究人员开展了新冠病毒感染组与Mock对照组样本间的基因定量与差异表达分析。本数据集筛选了错误发现率（False Discovery Rate, FDR）P值小于0.05的差异表达基因并上传至此。