A genetic system for tissue-specific inhibition of cell proliferation
收藏干细胞与再生医学数据中心2021-09-08 更新2024-03-06 收录
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We obtain multi-genotype mice through mouse mating, and induce tamoxifen at designated time points according to the purpose of the experiment, so that CreER enters the nucleus, and Cre-Loxp homologous recombination occurs, thereby driving the reporter gene or P21 gene overexpression. During the experiment, we randomly group the mice for injury induction, and each group of experiments has 3-5 biological replicates. We use Zeiss (V16) stereoscopic fluorescence microscope to perform fluorescence imaging of whole tissue specimens. We use cryomachine (Thermo) for frozen section. We use acquire fluorescence imaging of tissue sections by Olympus (FV1200) and Nikon (A1) confocal microscopes. We use StepOnePlus Real-time PCR System (Applied Biosystems) performs RT-PCR. Image experiment results are analyzed by Image J; data experiment results are analyzed by Prism.
提供机构：
中国科学院上海生物化学与细胞生物学研究所
创建时间：
2021-09-08
