Transcriptome data of the testes in obese mice

The aim of this study was to determine whether increasing NAD+ levels in an HFD-induced mouse model of obesity would improve spermatogenesis and, if so, to explore the underlying mechanisms. We found that supplementation with NMN, an NAD+ precursor, decreased body weight, improved lipid metabolism, and ameliorated sperm quality in obese mice. Importantly, we found that NMN treatment decreased acetylation of LDHC, a testis-specific lactate dehydrogenase, thereby increasing SCs lactate and NAD+ production, enhancing the expression of tight junction proteins by SCs, and improving spermatogenesis in these obese mice. Our findings provide new insight into the mechanisms responsible for reduced fertility in men and suggest potential treatment targets.