Global subcutaneous white adipose tissue (scWAT) transcript data from fasted male BXD strains on chow or high fat diet. Mus musculus

Transcript data from subcutaneous white adipose tissue (scWAT) from fasted-state male BXD strains on chow or high fat diet We used microarrays to compare the subcutaneous white adipose tissue (scWAT) expression differences across the BXD strain family and across two diverse diets Overall design: 29-week-old male mice were fasted overnight (6pm-9am), anesthetized under isoflurane, and perfused. After about five minutes (towards the end of the tissue collection pipeline), subcutaneous white adipose tissue was weighed then snap-frozen in liquid nitrogen. Later, the tissue was taken from a -80 freezer for RNA extraction from all individuals. Each dietary and strain cohort consisted of ~5 animals which were prepared independently. These indendent RNA samples were then then pooled into single dietary and genetic cohorts (as shown here) evenly by µg RNA as measured by Nanodrop. These pools were cleaned up with RNEasy, then frozen again in -80. Samples were then shipped from Lausanne, Switzerland to Memphis, TN, where the Affymetrix arrays were run. Tissues were in the -80 freezer approximately 1-2 years before RNA was prepared. RNA (post pooling by cohort, but pre-RNEasy cleanup) were in the freezer approximately 2.5 years before microarrays were run.