De novo assembly and transcriptome functional annotation of liver from five tropical bat species. Artibeus jamaicensis, Mormoops megalophylla, Myotis keaysi, Nyctinomops laticaudatus and Peropteryx macrotis

In this study we have generated whole transcriptomes from liver tissue in five species of tropical bats classified into five differentfamilies: Artibeus jamaicensis (F. Phyllostomidae), Mormoops megalophylla (F. Mormoopidae), Myotis keaysi (F. Vespertilionidae), Nyctinomops laticaudatus (F. Molossidae) and Peropteryx macrotis (F. Emballonuridae). This is the first report of transcriptome assembly by RNA-seq in these species, except for A. jamaicensis. HiSeq 4000 multiplex sequencing generated a total of 403 million paired-end reads of 101 bplength, sequencing depth ranged between 30 to 69 million paired end reads of which 99% were used for de novo assembly. Final non-redundant transcripts consisted of 685,849 for A. jamaicensis, 469,620 for M. megalophylla, 456,572 for M. keaysi, 617,036 for N. laticaudatus and 634,439 for P. macrotis. We were able to recover between 65 and 75% of vertebrates single copy orthologues, suggesting that the assembled transcriptomes have a good level of completeness.