RNA-seq of primary mouse hepatocyte, primary mouse adipocyte, and C2C12 cell line treated with olanzapine and/or either calcifediol or calcitriol

Olanzapine is considered to induce dyslipidemia as an adversary effect in clinical practice. Critically, raised low-density lipoprotein levels and lowered high-density lipoprotein levels in the blood of patients are detected. The present study utilized three independent sources of clinical big data to analyze the drugs potent to prevent olanzapine-induced dyslipidemia, and vitamin D was found to be effective. To clarify the molecular mechanism of the influence of olanzapine and vitamin D, the culture of three cholesterol metabolism-related cells of mice, primary hepatocyte, primary adipocyte, and C2C12 cells (myocyte) were introduced to treat with olanzapine and vitamin D primary metabolites like calcifediol and calcitriol. RNA-seq was performed on these three types of cells to uncover the direct effects of olanzapine and vitamin D in cholesterol metabolism. mRNA profiles of primary mouse hepatocytes, primary mouse adipocytes, and C2C12 cell line under vehicle(DMSO), olanzapine, olanzapine + calfediol, or olanzapine + calcitriol treatment