RNAseq analysis of PS+ vs. PS- effector CD8 T cells sorted from spleen of LCMV-Armstrong infected mice

We report the transcriptional profile of phosphatidylserine (PS) positive and PS negative CD44+CD62L- effector CD8+ T cells from LCMV-Armstrong infected mice sorted from spleens on day5 amd day10 after infection. PS was stained in vivo by injection of MFG-E8-eGFP. PS+ cells acquire PS through binding of extracellular vesicles (EVs). Binding of EVs by effector CD8+ T cells is strongly enhanced during LCMV infection and peaks between day5 and day10 post infection. Effector T cell gene signature is increased in EV+ CD8+ T cells compared to their EV- counterparts. Mice were infected ip with 2x10^5 pfu LCMV Armstrong. On day 5 and day10 of infection, 100µg MFG-E8-eGFP were administered iv to stain phosphatidylserine positive cells (mainly apoptotic cells and cells associated with EVs). 1h after MFG-E8-eGFP administration mice were sacrificed and CD44+CD62L- CD8+ T cells sorted from the spleen using a FACS AriaIII. Cells were lysed in Trizol and subjected to RNAseq analysis.