Sequencing of Dothistroma septosporum chrysovirus-1. undefined

A new double-stranded (ds) RNA mycovirus has been identified in isolate Ds752-1 of the phytopathogenic fungus Dothistroma septosporum, the causal agent of Dothistroma needle blight, also known as red band needle blight or pine needle blight. Dothistroma septosporum chrysovirus-1 (DsCV-1) constitutes a new member of the Alphachrysovirus genus in the Chrysoviridae family. The DsCV-1 genome comprises four dsRNA elements designated 1, 2, 3 and 4 from largest to smallest. DsRNA1 encodes an RNA dependent RNA polymerase (RdRP) most similar to the RdRP of Erysiphe necator associated chrysovirus-3. DsRNA2 potentially encodes two hypothetical proteins, one small of no homology to known proteins and one large with significant homology to the alphachryso-P3 of other alphachrysoviruses. DsRNA3 and dsRNA4 encode a coat protein (CP) and a putative cysteine protease, respectively. DsCV-1 constitutes the first report of a mycovirus infecting the fungus D. septosporum and the first member of the Chrysoviridae family found to possess a genomic dsRNA potentially encoding more than one protein.