Labcode 7- HIV neutralization Protocol PBMC Neutralization assay
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https://doi.org/10.7910/DVN/EKISQY
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资源简介：
This document describes the isolation of Peripheral Blood Mononuclear Cells (PBMCs) from buffycoat, the stimulation of PBMCs by PHA, and the determination of the neutralization sensitivity of HIV-1 strains using a PBMC neutralization assay. Two different protocols are in use for the PBMC neutralization assay, depending on whether viruses are tested for sensitivity to a polyclonal (serum) or monoclonal (antibody) neutralizing agent.
创建时间：
2016-03-10
