Liquid culture fluorescence data of the GFP insertion constructs expressing four test proteins with progressively decreasing solubility at 37°C.

aProtein #1 sulfite reductase (dissimilatory subunit); protein #2 (translation initiation factor), protein #3 (3-hexulose 6-phosphate synthase), and protein #4 (polysulfide reductase subunit). bFraction of non-fusion protein soluble expressed in E. coli at 37°C, as determined by SDS-PAGE. cScaffolding topology of GFP folding reporter. dType of GFP domain used in reporter, SF = superfolder GFP, FR = folding reporter GFP. eThe measured fluorescence (488 nm excitation, 520 nm emission) normalized by dividing by the optical density at 600 nm.