Paired-end RNA-seq data from 12 ascites-derived organoid samples of ovarian cancer patients

This dataset contains paired-end RNA sequencing data from 12 ovarian cancer patient Ascites derived organoid samples. Samples were collected, processed, and sequenced to study transcriptomic changes associated with Doppel expression, epithelial-to-mesenchymal transition, and tumor dissemination. Each sample consists of two raw FASTQ files: - **R1**: Forward reads (`_1.fq.gz`) - **R2**: Reverse reads (`_2.fq.gz`) File Naming Convention Files are as follows: ‘PtXX_1.1.fq.gz’ → Forward read (R1) for patient XX ‘PtXX_1.2.fq.gz’ → Reverse read (R2) for patient XX Example: - Pt24AsOr1_1.fq.gz→ Forward reads from patient 24 - Pt24AsOr1_2.fq.gz→ Reverse reads from patient 24 Sample List: Please see the metadata file Data Summary · Total samples: 12 (24 FASTQ files; paired end). · Organism: Homo sapiens. · Tissue source: Ovarian tumor ascites-derived cells. · Sequencing platform: Illumina NovaSeq 6000. · Read length: 2 × 150 bp. · Data type: Raw FASTQ (gzip-compressed) Methods Summary RNA was extracted from ascites-derived organoids using combined Trizol and Qiagen RNase easy mini kit. RNA quality was assessed using bioanalyzer, and libraries were prepared using TruSeq Stranded mRNA Library Prep Kit. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired end reads of (2 × 150 bp). Usage Notes · Use the forward (R1) and reverse (R2) files together for alignment and downstream analyses. · FASTQ files are untrimmed; adapters and low-quality bases should be removed prior to analysis. · Recommended Aligner: HISAT2. Contact For questions regarding this dataset, please contact: Dr. Taslim A. Al-Hilal, Associate Professor, Department of Molecular Pharmaceutics, University of UTAH, Salt Lake City, UTAH, Email: [e-mail]