Stable knockdown of PLAGL2 in hepatocellular carcinoma cells

Pleomorphic adenoma gene like-2 (PLAGL2), along with PLAG1 and PLAGL1, belong to PLAGL gene family of zinc-finger transcription factors, PLAG proteins are highly homologous, and with the structures of the seven C2H2 zinc finger domain on the N-terminal. PLAG proteins recognize similar GC rich consensus DNA-binding sequences, that these family members function as regulators of transcription target genes and pathways. PLAGL2 has been shown to have both oncogenic and tumor suppressor activities, PLAGL2 is mainly consided as a transcription factor, other functions which may contribute to its oncogenic potential are not clear. So it is meaningful to study the role of PLAGL2 in different diseases. To our knowledge, there is no report about the PLAGL2 in the development process of HCC. mRNA profile of Hepatocarcinoma cell line HCC-LM3 with shctrl and shPLAGL2 were generated by deep sequencing