Single-nucleus RNA seq of murine renal tumors and lung metastases generated by RENCA cells overexpressing Interleukin-34

Murine renal carcinoma RENCA cells were injected in BALB/c mice to generated either primary tumours (orthotopically) or lunge metastases (through tail vein injection, or directly forming from primary tumors). Samples were snap-frozen and processed for single nucleus isolation and RNA seq analysis. Tumor cells expressed Interleukin-34 (IL34-OE) or an empty vector as control (Ctrl). The goal is to finely dissect tumor microenvironmental changes occurring in an IL34-enriched tumor microenvironment, either in primary tumors or lung metastases.