Gene expression profile at a single cell level of differentiating J1 mouse embryonic stem cells cocultured with OP9 cells

The OP9 coculture system is widely used to differentiate pluripotent stem cells into mesodermal lineages. We used single cell RNA sequencing to analyze differentiation trajectories and heterogeniety of differentiating pluripotent stem cells using the OP9 coculture system. J1 mouse embryonic stem cells were cocultured with mitomycin-treated OP9 cells for 5 days. Undifferentiated J1 mouse embryonic stem cells were used as a control group.