Major populations of equine PBMC

Phenotype equine PBMC by flow cytometry for comparison to single cell RNA-Sequencing. Panel M was used to determine the proportion of T cells, B cells, monocytes, NK-like cells (CD3+CD16+), and alternatively activated monocytes (CD14+CD16+). Panel T was used to determine CD4+ and CD8+ T cells and replicating Ki67+ T cells. Panel B1 and Panel B2 were used to characterize Tbet expression in B cells and co-expression of T bet with other markers. Conclusion: Flow cytometry and scRNA-Seq quantification of these cell populations showed very high correlation. Horses have a markedly high proportion of Tbet+ B cells. Seven biological replicates with different ages, sexes, and breeds were used. Single color compensation controls were included.