Data underlying the publication: “Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation”

The research objective was to quantify at the single-molecule level the motion of fully in vitro reconstituted CMG, the eukaryotic replicative helicase. <br> This was achieved by using a combination of optical tweezers and confocal fluorescence microscopy. The position along DNA molecules held in an optical trap of fluorescently labeled CMG was tracked over time, and different motion parameters were studied (e.g. diffusion constants and velocities) under different biochemical conditions.

本研究的核心目标是在单分子水平上定量表征完全体外重构的真核复制解旋酶（CMG）的运动行为。
本研究通过结合光镊与共聚焦荧光显微镜技术实现了这一目标。研究人员对结合于被光阱捕获的DNA分子上的荧光标记CMG的位置进行了实时追踪，并在不同生化条件下对其多项运动参数（如扩散常数与运动速率）开展了分析。