qRT-PCR Ct values and relative titers of soybean cyst nematode (SCN) viruses within SCN research populations.

Mean qRT-PCR cycle threshold (Ct) values are shown for SCN nyami-like virus (NLV), SCN bunya-like virus (BLV), and internal SCN control (HgFAR1 and GAPDH). Relative viral titers were calculated and log2 adjusted by comparison against internal controls using a modified Pfaffl method [7,26,27]. Negative log2 relative titers denote a value below the internal control; whereas, positive values represent titers higher than the internal controls. ND (not detected) demonstrates that virus was not found in the sample. Experiments were conducted in technical triplicates and standard deviations are shown.