Snapgene Files, QC files (plasmid sequencing results), and service reports from ABM

The heterogeneity of their structure and composition hinders the application of medical therapies and diagnosis. To address this issue, our approach leverages genetic engineering to mark exosomes specifically, allowing for their precise identification and isolation from a complex biological pool of heterogenous EVs. The isolated cell-specific exosomes were characterized by electron microscopy, Nanoparticle tracking analysis, antibody exosome array assay, and other reliable techniques mentioned in the protocol. The labeling and isolation of exosomes typically spans 2-3 days and is designed to be accessible to researchers with fundamental laboratory competencies. This protocol facilitates the study of exosome-mediated communication by enabling the isolation of exosomes from either individual cell types or multiple cell types in combination. Most of the experiments with the protocol have used mouse wound-edge skin tissue, but the protocol can also be applied to other tissues to isolate exosomes.