Detection of primary infected cells in dispersed TGEs at 2 dpi.

aTGEs were infected either in the axonal or ganglion compartment (AC, GC).bCultures were infected with HSV-1 17syn+ in the presence of 50 µg/ml ACV, harvested at 2 dpi and dispersed.cGenome levels per dispersed TGE were quantified by qPCR (mean of three experiments).d,eHSV-1-positive cells and neurons per dispersed TGE were detected and quantified by immunofluorescence with a HSV-1-specific rabbit antiserum (d), a monoclonal antibody against the 200 kD neurofilament marker (e) and DAPI (d,e) (mean of three experiments).