High spatial resolution multi-omics atlas sequencing of mouse embryos

Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution. Overall design: Applying new approach – microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10µm, 25µm, 50µm).