Values of Area under the survival curve (AUC) of strain BY4742 cultured in different medium composition.

n.d. – not determined. Cells were grown in SC media supplemented with low (Low-AA) or high (High-AA) concentrations of auxotrophy-complementing amino acid and with 0.01%; 0.1%; 0.5% or 1% (NH4)2SO4 for 72 hours; or cells were grown in SC media until O.D. 1–1.5, harvested and resuspended in Nitrogen-starvation medium (N-) or in amino acid-starvation medium (aa-) for 24 hours. The aging assays were performed by resuspending cells from the different culture conditions in their respective exhausted medium, exhausted medium pH 7, or in 0.5 and 1% (NH4)2SO4, pH 7.