Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array. Homo sapiens

Indel analysis of Cpf1 in HEK293FT cells and RNA sequencing of Cpf1 array processing in E coli. Targeted loci

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To demonstrate Cpf1 can be engineered to modify PAM recognition, broadening the targeting range in rice.

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Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion

Mutations and genetic alterations are often sequentially acquired in various biological and pathological processes, such as development, evolution, and cancer. Certain phenotypes only manifest with pr

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Multiplex gene editing by CRISPRâCpf1 using a single crRNA array

Multiplex gene editing by CRISPRâCpf1 using a single crRNA array