data used to create main figures for: Park et. al., Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA, BMC Biol, 2023.
We characterize genetic determinants of human cardiomyocyte polypolidization and CCNB1 insufficiency using pooled CRISPR screen methods CCNB1-GFP iPSCs were transduced with lentiviral Cas9 and librari
Raw FASTQ data for [Hawkins JS, Silvis MR, et. al. 2020]. Samples reflect amplified identifier sequences from B. subtilis and E. coli strain collections before and after competitive growth experiments
We compared changes in sgRNA abundance between T24 cells challenged and unchallenged by CD8+ T cells, and then performed negative screening (p < 0.01, log2Fold change < −1.0, and good sgRNAs ≥ 5). (X