Colonic cytokines and chemokines in DNBS colitis

Abdominal pain in irritable bowel syndrome and inflammatory bowel disease is thought to be driven by processes that sensitize sensory nerves innervating the gut. How sensory nerves become sensitized is not clear, but their terminals in the gut are surrounded by enteric glia. Here, we tested the hypothesis that intercellular enteric glia-to-nociceptor signaling contributes to visceral hypersensitivity during inflammation. In vivo and in vitro models of acute inflammation were used in combination with protein and RNA labeling, and cellular assays of activity and mediator release. Mechanisms of interaction between glia and nociceptors were studied using Trpv1Cre;GCaMP5gtdT;GFAP-hM3Dq mice, in which glial activity is controlled by chemogenetics while simultaneously recording nociceptor activity using calcium imaging. Mice lacking glial connexin-43 were used in combination with visceromotor reflex recordings to disrupt glial intercellular signaling and study its impact on visceral sensitivit..., Whole colon samples were collected from control and DNBS treated mice at 6 hours, 48 hours, and 3 weeks. Tissue was processed for multiplex immunoassay as previously described (50). Colonic samples were homogenized in a Tris-buffered saline Tween buffer solution to extract protein. After, homogenates were centrifuged at 10,000g for 10 minutes at 4°C. Cytokine/chemokine levels were evaluated using bead-based multiplex immunoassays (Eve Technologies, Calgary, AB, Canada)., , # Colonic cytokines and chemokines in DNBS colitis

[https://doi.org/10.5061/dryad.jwstqjqgr](https://doi.org/10.5061/dryad.jwstqjqgr)

Analysis of colonic cytokine and chemokine levels during the the time course of DNBS colitis in male and female mice. Concentrations reflect levels in samples of whole colon in healthy controls, during the acute, proinflammatory phase of colitis (6 hours and 48 hours) and following resolution (3 weeks). Colon homogenates were analyzed Eve Technologies (Calgary, AB) using a commercial bead-based multiplex immunoassay.

## Description of the data and file structure

The file contains a spreadsheet of raw data. Individual cytokines are labeled in columns. Samples identified under \"Treatment\" represent whole colon homogenates obtained from animals at 6 hours post DNBS (6h DNBS), 48 hours post DNBS (48h DNBS), or 3 weeks post DNBS (3 3 DNBS). Animal sex is indicated by \"F\" for female and \"M\" for male. \"FI\" signifies the Fluorescence Intensity of the bead pop...