Effect of Overexpression of VIRMA on m6A-methylated mRNAs in breast cancer cells

To identify m6A-regulated mRNA targets affected by VIRMA overexpression we compared m6A peak abundance measured via m6A RNA immunoprecipitation sequenc-ing in SKBR3 cells overexpressing full-length VIRMA and eGFP control m6A-RIP-sequencing was performed according to the recently published “Refined RIP-seq” with several modifications. . 5 μg total RNA was fragmented into ~200 nucleotide long fragments via incubation with ZnCl2 at 70˚C for 3 minutes. Fragmented RNA was precipitated, and the RNA size distribution was assessed using RNA 6000 Nano Bioanalyzer kit