Deep mutational scanning of mutant RBD libraries from SARS-CoV-2 variants and sarbevoviruses to detect mutations that reduce binding of polyclonal serum as well as mAbs.

We performed deep mutational scanning of the spike receptor-binding domain (RBD) from SARS-CoV-2 variant and saerecovirus viral lineages using yeast display to measure the effects of all amino-acid mutations on various on binding of antibodies or polyclonal plasma/sera.