Platelets induce endothelial cell mitochondrial dysfunction in myocardial infarction

Coronary endothelial dysfunction playsa key role in the pathogenesis of cardiovascular events. In the setting of myocardial infarction (MI), activatedplatelets release prothrombotic and proinflammatory factors, contributing to vascular injury and dysfunction.However, the effect of platelets on coronary endothelial function during MI is not fully understood. We investigated the effect of platelets from patients with MI on cultured coronary endothelial cells in vitro. Overall design: Human coronary artery endothelial cells (HCAECs) from one donor were treated with platelet releasate from patients with myocardiol infarction or controls for 6 hours. Another group of HCAECs were treated with CCL3 (100 ng/mL) or PBS for 6 hours.