Genome-wide maps of chromatin state in mouse Th17 cell [ChIP-seq]

We applied ChIP-seq technology for mapping chromatin modification in Th17 cells. We find that acetylations on histone H3 lysine 27, an active enhancer mark, significantly overlap with open chromatin as defined by ATAC-seq. These results were used to define putative super enhancer loci in Th17 cells. Our study shows that super-enhancer mediated gene transcription is important for Th17 effector functions. Examination of histone H3 lysine 27 acetylation in two independent biological replicates of cultured Th17 cells. Input genomic DNA from each biological replicate were used for MACS peak call analysis.