scNanoSeq-CUT&Tag: a long-read single-cell CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells

We present scNanoSeq-CUT&Tag ,which is based on a long-read sequencing platform. In six human cell lines, we identified regions regulated by H3K4me3,H3K27ac,H3K36me3,H3K27me3,H3K9me3,CTCF and RAD21 modifications. Long fragments (about 4-5Kb) of single-cell ATAC-seq library were enriched and sequenced by Oxford Nanopore Technologies platform. Ends of long CUT&Tag fragments are regarded as chromatin modification signal in downstream analysis.