five

WT and Aire-KO B6 MEChi RNAseq profiling

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE177062
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Aire-sensitive genes exhibit a weak inclusion of alternative splicing events Total RNA isolated by Trizol extraction from MHC-II high mTECs (pool of mice) was used to generate poly-A-selected transcriptome libraries using the TruSeq stranded mRNA library Prep following the manufacturer's protocols. Paired-end (2 × 100 bp) sequencing was performed using the Illumina HiSeq2000 machine.
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2022-01-24
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