Asparagine alleviates naphthalene-induced lens opacity in SRA cells

Cataract is characterized by lens opacity, often leading to vision loss. The primary clinical treatment is lens replacement, which usually yields good results. Although cataract surgery is a common ophthalmic treatment, it still carries surgical risks, such as infection, bleeding, and dislocation of the intraocular lens. Therefore, researching drugs that can delay or treat cataract holds significant social and scientific importance. This study found that asparagine can improve lens opacification in rats exposed to naphthalene. Consequently, in vitro, SRA01/04 cells were treated with the naphthalene metabolite 1,2 - dihydroxynaphthalene (1,2 - DHN), and RNA - sequencing technology was employed to investigate the cellular mechanisms underlying the therapeutic effects of asparagine.Through systematic experiments and analyses, we have dissected the cellular changes following treatment with 1,2 - DHN, comprehensively presenting the molecular network affected thereby. Simultaneously, we have also paid particular attention to the signaling pathways activated by Asn during this process. RNA-seq was used to detect the differences in SRA01/04 cells among the normal group, the normal group supplemented with asparagine, the 1,2-DHN treatment group, and the 1,2-DHN plus asparagine treatment group after 24 hours of treatment.