Transcription profiling of non squamous cell lung cancer cell lines to investigate sensititivity to gefitinib and predict this in previously untested cell lines

Eleven NSCLC cell lines with widely divergent gefitinib sensitivities were compared using gene expression. Genes associated with gefitinib response were used to classify additional NSCLC lines with unknown gefitnib sensitivity. A subset of the test set data was tested for gefitinib sensitivity, and results correlated strongly with the gene expression-based predictions; All eleven training set lines, and seven test set lines had both HGU133A and B chips done, while other test set lines had only HGU133As. Experiment Overall Design: Baseline (unstimulated) gene expression was measured in a large panel of NSCLC cell lines.