cDNA expression profiles of +7 DPA wild-type upland cotton (Gossypium hirsutum cv. Xuzhou142) ovules over the one-year cycle

A cDNA library from 0-10 day post anthesis cotton ovules was established to study genes expressed in cotton ovule during initiation and quickly elongation period. We randomly sequenced over 100,000 ESTs from this library and acquired a gene pool of more than 28,000 UniESTs. The cotton UniESTs were then PCR-amplified and printed onto microarray. This array is comprised of about 28000 high-quality cotton cDNAs (with average length>750bp) and external controls. To study the different growth potential of cotton fibers in a one-year cycle, we then hybridized the array with RNA samples derived from +7 DPA wild-type upland cotton fibers in four different seasons, respectively. Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: spring-Cy5 common_reference-Cy3 Set 1 (dye swap): common_reference-Cy5 spring-Cy3 Set 2: spring-Cy5 common_reference-Cy3 Set 2 (dye swap): common_reference-Cy5 spring-Cy3 Set 3: spring-Cy5 common_reference-Cy3 Set 3 (dye swap): common_reference-Cy5 spring-Cy3 Set 4: spring-Cy5 common_reference-Cy3 Set 4 (dye swap): common_reference-Cy5 spring-Cy3 GSM419228, GSM419333, GSM419334, GSM419335, GSM419336, GSM419337, GSM419338, GSM419340 Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: summer-Cy5 common_reference-Cy3 Set 1 (dye swap): common_reference-Cy5 summer-Cy3 Set 2: summer-Cy5 common_reference-Cy3 Set 2 (dye swap): common_reference-Cy5 summer-Cy3 Set 3: summer-Cy5 common_reference-Cy3 Set 3 (dye swap): common_reference-Cy5 summer-Cy3 Set 4: summer-Cy5 common_reference-Cy3 Set 4 (dye swap): common_reference-Cy5 summer-Cy3 GSM419341, GSM419343, GSM419345, GSM419346, GSM419348, GSM419349, GSM419351, GSM419352 Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: autumn-Cy5 common_reference-Cy3 Set 1 (dye swap): common_reference-Cy5 autumn-Cy3 Set 2: autumn-Cy5 common_reference-Cy3 Set 2 (dye swap): common_reference-Cy5 autumn-Cy3 Set 3: autumn-Cy5 common_reference-Cy3 Set 3 (dye swap): common_reference-Cy5 autumn-Cy3 Set 4: autumn-Cy5 common_reference-Cy3 Set 4 (dye swap): common_reference-Cy5 autumn-Cy3 GSM419428, GSM419429, GSM419430, GSM419431, GSM419432, GSM419433, GSM419434, GSM419435 Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: winter-Cy5 common_reference-Cy3 Set 1 (dye swap): common_reference-Cy5 winter-Cy3 Set 2: winter-Cy5 common_reference-Cy3 Set 2 (dye swap): common_reference-Cy5 winter-Cy3 Set 3: winter-Cy5 common_reference-Cy3 Set 3 (dye swap): common_reference-Cy5 winter-Cy3 Set 4: winter-Cy5 common_reference-Cy3 Set 4 (dye swap): common_reference-Cy5 winter-Cy3 GSM419436, GSM419437, GSM419438, GSM419439, GSM419440, GSM419441, GSM419442, GSM419443