Treatment with LPAAT-β siRNA results in inhibition of phosphorylation of mTOR effector proteins 4E-BP1, S6K, and AKT.

AsPC-1 (A) and MiaPaCa2 (B) cells were treated for 72 hr with 25 nM LP-1, LP-4, non-targeting siRNA (nt), or a mock transfectant (m). Western blots were done with the indicated antibodies to mTOR effector proteins and mTOR-specific phosphorylation sites, as well as a Vinculin loading control. The graphs show densitometric quantitation of the phosphorylated bands normalized to both Vinculin and their corresponding whole protein. Results are representative of three independent experiments.